Nanotechnology Images

Grade 23 ELI Ti alloy

SEM images of Grade 23 ELI Ti alloy Shot Peened test surfaces at 5000x (A,C,E,G) and 50,000x magnification (B,D,F,H). ELI Ti (A,B); Nanorough acid etched (C,D); ELI Ti with CaP (E,F); Nanovis Bioceramic Nanotubes (G,H). CaP treatment present on (C,D,G,H).

Osteoblast Mineralization
Day 21 (100x Magnification)

Alizarin Red staining of calcium deposits on human osteoblast differentiation cultures after 21 Days (A) ELI Ti control surface, (B) Nanorough acid etched ELI Ti, (C) CaP treated ELI Ti, (D) Nanovis Bioceramic Nanotubes. 100x magnification scale bar represents 50 microns.

Mesenchymal Stem Cell Mineralization
Day 21 (100x Magnification)

Alizarin Red staining of calcium deposits on human MSC differentiation cultures after 21 Days (A) ELI Ti control surface, (B) Nanorough acid etched ELI Ti, (C) CaP treated ELI Ti, (D) Nanovis Bioceramic Nanotubes. 100x magnification scale bar represents 50 microns.

Residual Bone

Flakes of residual bone on the surface of extracted pedicles screws in image A and B demonstrates that the bone failed while the bioceramic nanotubes remained attached to the screw. The bone matrix can be seen in image C and the collagen rich bone matrix can be seen attached to the nanotubes in D.

SEM images of residual bone on Nanovis’ Bioceramic nanotube pedicle screws after extraction from horse bone after 13 weeks of implantation. Magnifications: 30x (A), 70x (B), 10,000x (C), 50,000x (D).